A thaumatin-like protein gene fromBasrai banana was cloned and expressed in Escherichia coli. Amplified gene product was cloned\ninto pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced\ninto E. coli BL21. Maximum protein expression was obtained at 0.7mM IPTG concentration after 6 hours at 37âË?Ë?C. Western blot\nanalysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological\nagent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against\nA. fumigatus; however combined effect of both agentsmaximized activity against the pathogen. Docking studies were performed to\nevaluate the antimicrobial potential of TLP against A. fumigatus by probing binding pattern of antifungal protein with plasma\nmembrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of\nantifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in\ncontrolling the ice crystal formation in frozen yogurt was also studied.Thescope of this study ranges fromcost effective production\nof pharmaceutics to antifreezing and food preserving agent as well as other real life applications.
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